摘要
以爱玉子的叶片为材料,对CTAB分离总DNA的方法进行了改进,即在提取液中加入β-巯基乙醇、PVP,将CTAB的浓度提高到3%,用氯仿-异戊醇取代酚进行抽提。结果表明,改进后的方法可有效地去除杂质,防止材料褐化,获得的DNA样品OD260nm/OD280nm比值为1.8左右,质量好且产率较高,能完全满足SRAP分析及限制性内切酶酶切的要求。
This paper explored an improved CTAB method to extract high-quality genomic DNA from the leaves of Ficus awkeotsang Makino, which was the application of β-SH （mercapto） ethanol and PVP, and increasing the concentration of CTAB up to 3 %, and substituting chloroform-isoamylol for phenol. The research results showed that this modified method can effectively elimi nate impurity and prevent DNA samples from turning brown. High-quality and high-yield genomic DNA with OD260nm/OD280nm approximate 1.8 was obtained, and it was fully suitable for SRAP analysis and restriction enzyme digestion.
出处
《福建林业科技》
北大核心
2008年第3期48-50,66,共4页
Journal of Fujian Forestry Science and Technology
基金
福建省科技厅重点资助项目(2007S0015)
福建省自然基金项目(B0610029)
福建省大学生创新性实验计划项目(Fjnu2008-012)
关键词
爱玉子
DNA提取
SRAP
限制性内切酶酶切
Ficus awkeotsang Makino
DNA extraction
SRAP
restriction enzyme digestion
