火焰卫矛组织培养及植株再生研究

Tissue culture and plantlet regeneration of Euonymus alatus 'compacta'

以火焰卫矛成熟胚培养长出的子叶为外植体进行组织培养及植株再生研究,建立了火焰卫矛再生体系,成熟胚培养基为WPM+TDZ 0.5 mg.L-1;子叶愈伤组织诱导和不定芽分化培养基为WPM+6-BA 6 mg.L-1+IBA 0.2 mg.L-1;生根培养基为1/2MS+NAA 0.2 mg.L-1+活性碳0.5 g.L-1。

The cotyledon from mature embryo of Euonymus alatus‘compacta＇ was used as the explant for studying the tissue culture and the plandet regeneration. The results indicated that the medium for mature embryo was WPM ＋ TDZ 0.5 mg·L^-1 the medium for callus induction from the cotyledon, and the medium for bud differentiation were WPM ＋ 6-BA 6 mg·L^-1 ＋ IBA 0.2 mg·L^-1, the medium for rooting of adventitious buds was 1/2MS ＋ NAA 0.2 mg·L^- 1 ＋ active carbon 0.5 g·L^-1. These provide fundamentals for rapid propagation and genetic engineering of Euonymus alatus ‘ compacta＇.