弥漫性脑损伤大鼠颅内压及脑灌注压的变化及DCLHb治疗作用

ICP and CPP levels in a rodent model of diffuse brain injury with secondary insults

目的：研究大鼠加速性弥慢性脑损伤合并低血压及脑缺血、缺氧时，颅内压（ＩＣＰ）及脑灌注压（ＣＰＰ）变化与双阿斯匹林联偶血红蛋白液（ＤＣＬＨｂ）治疗作用。方法：２４只ＳＤ大鼠随机分为假手术对照、脑损伤并低血压与脑缺血及治疗三组。采用Ｍａｒｍａｒｏｕ大鼠加速性弥慢性脑损伤模型，抽血及颈动脉结扎造成低血压及脑缺血、缺氧。所有动物均气管内插管并实施同步生理监护。结果：伤后４小时，与假手术组对比，合并低血压与脑缺血组出现ＩＣＰ增高及ＣＰＰ降低（Ｐ＜０．０５），ＤＣＬＨｂ治疗组二者接近正常。结论：合并低血压与脑缺血组出现ＩＣＰ增高及ＣＰＰ降低提示低血压或脑缺血参予脑损害的加重过程，ＤＣＬＨｂ则可能通过提高ＣＰＰ发挥脑保护作用。

Objective:In the present study the changes of intracranial pressure (ICP) and cerebral perfusion pressure (CPP) in a rodent model of impact acceleration diffuse brain injury with hypotention and hypoxia were observed and the effect of diaspirin cross linked homoglobin sloution (DCLHb) also investigated. Methods: Twenty four male SD rats were randomized into sham, head injury with secondary insults and injury with insults followed by DCLHb administration three groups. Marmarou's rodent model of impact acceleration diffuse brain injury was coupled with hypotention made by blood with drawing and hypoxia made by carotid arteries occlusion. All animals were intubated and physiologically monitored minute by minute. Results: Compared with that of sham group there was a increased ICP and a decreased CPP level in head injury with secondary insults group and both ICP and CPP normalized in injury with insults followed by DCLHb administration at 4 hours postimpact. Conclusion: The rise in ICP and reduction in CPP in head injury with secondary insults rats suggest that secondary insults are involved in the aggravation of primary brain damage. And it is hypothesized that DCLHb may exert its protective properties through increasing CPP level in injured brain.