GM-CSF诱导红白血病细胞向树突状细胞分化及其抗原提呈功能的研究

The dendritic cell differentiation and antigen presenting function of the erythroleukemia cells induced by GM CSF

目的探讨粒细胞－巨噬细胞集落刺激因子（ＧＭ－ＣＳＦ）诱导小鼠红白血病细胞分化过程中，树突状细胞的产生及其抗原提呈功能的变化。方法采用流式细胞仪分析、电镜技术和４小时５１Ｃｒ释放法等，进一步观察了ＧＭ－ＣＳＦ的诱导分化作用。结果１００ｎｇ／ｍｌＧＭ－ＣＳＦ处理３天后的ＦＢＬ－３细胞，树突状细胞的特异性标志３３Ｄ１和ＮＬＤＣ１４５的表达阳性率显著升高；同时，ＭＨＣ－Ⅱ、Ｂ７－１、Ｂ７－２、Ｉ－ＣＡＭ－１、ＶＣＡＭ－１和ＣＤ４０表达水平均增加；扫描电镜显示ＧＭ－ＣＳＦ处理的ＦＢＬ－３细胞，表面出现许多树突状突起，透射电镜下可见细胞浆内有丰富的线粒体，核呈分叶状。同时，能够显著刺激同种异体Ｔ淋巴细胞增殖，促进ＩＬ－２的产生；可显著提高细胞毒性Ｔ淋巴细胞（ＣＴＬ）细胞对ＦＢＬ－３细胞的特异性杀伤活性。结论ＧＭ－ＣＳＦ诱导的红白血病细胞可向树突状细胞分化，本身具有抗原提呈功能。

Objective To investigate the generation of dendritic cells and the change of antigen presenting function during the differentiation of FBL 3 erythroleukemia cells induced by GM CSF. Methods The effects of GM CSF on the phenotype, ultrastructure and antigen presenting function of FBL 3 erythroleukemia cells were observed by FACS, electromicroscopy and 51 Cr release assay. Results After treatment with 100 ng/ml GM CSF for 3 days, the expressions of 33 D1 and NLDC 145 which are the specific markers on dendritic cells were increased significantly; MHC Ⅱ,B7 1,B7 2,ICAM 1, VCAM 1 and CD40 were also upregulated. The membrane of FBL 3 cells was changed into villous surface with dendritic projections. There were plenty of mitochondria in cytoplasm, the nucleus became lobulated. The GM CSF treated FBL 3 cells could apparently stimulate the proliferation of allogeneic T lymphocytes, induce the production of IL 2 and improve the specific cytotoxic activity of CTL on FBL 3 cells. Conclusion Erythroleukemia cells were induced to differentiate into the dendritic cells by GM CSF and obtained the antigen presenting function.