摘要
以6个罗勒品种为材料，研究不同激素（6-BA，KT，ZT，NAA）组合对植株再生的影响。结果表明在6种基因型中，甜罗勒的效果最好；罗勒不定芽诱导的最佳培养基组合为MS＋ZT 0．4mg／L＋KT 0．1mg／L＋6～BA 0．2mg／L＋NAA 0．02mg／L。罗勒不定芽伸长的最佳培养基组合为MS＋ZT 0．4mg／L＋KT 0mg／L＋6-BA 0．2mg／L＋NAA 0．04mg／L。适合于不定芽生根的不定根诱导培养基组合为1／2MS＋IAA 0．2～0．3mg／L。
Six basil cuhivars were used as the experimental materials and the effects of different combinations of phytohormones （6 - BA, KT, ZT, NAA） in orthogonal test on the plant regeneration of basil were studied. The results indicated that the best genotype was sweet basil among the 6 genotypes ; the optimal concentration for inducing adventitious buds was MS ＋ ZT 0. 4 mg/L ＋ KT 0.1mg/ L ＋ 6 - BA 0. 2 mg/L ＋ NAA 0. 02 mg/L ; Adventitious buds elongated well on MS ＋ ZT 0. 4 mg/L ＋ KT 0mg/L ＋ 6 - BA 0. 2 mg/L ＋ NAA 0.04mg/L. The optimized medium for inducing root was 1/2MS ＋IAA 0. 2 -0. 3 mg/L.
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
2008年第5期658-662,共5页
Journal of Yunnan Agricultural University:Natural Science
基金
云南省自然科学基金(2005C0023Q)
云南省教育厅青年科学研究基金(04Y559B,07Y11682)
关键词
罗勒
组织培养
植株再生
正交实验
Ocimum basilicum L.
tissue culture
plant regeneration
orthogonal test
