IL-2和(或)IL-3基因治疗对大剂量化疗后小鼠免疫功能恢复的影响

The effect of IL 2 and IL 3 gene therapy in mice on immunological recovery receiving high dose chemotherapy

目的探讨ＩＬ－２、ＩＬ－３对化疗后小鼠免疫功能恢复的影响。方法正常小鼠腹腔注射大剂量环磷酰胺２４小时后，直接腹腔注射ＩＬ－２重组腺病毒（Ａｄ－ＩＬ－２）和（或）ＩＬ－３重组腺病毒（Ａｄ－ＩＬ－３），观察小鼠腹腔巨噬细胞数量、杀伤活性、Ｉａ抗原表达、脾细胞增殖及ＮＫ细胞杀伤活性。结果腹腔注射Ａｄ－ＩＬ－３组小鼠，腹腔巨噬细胞数量明显增加，杀伤活性及Ｉａ抗原表达显著增强。腹腔注射Ａｄ－ＩＬ－２组小鼠，脾细胞增殖能力显著升高，ＮＫ细胞杀伤活性增强，但对小鼠腹腔巨噬细胞无激活作用。联合应用Ａｄ－ＩＬ－２及Ａｄ－ＩＬ－３组小鼠，腹腔巨噬细胞、脾细胞、ＮＫ细胞均被激活。结论腹腔直接注射Ａｄ－ＩＬ－２和Ａｄ－ＩＬ－３可促进化疗后小鼠免疫功能的恢复。

Objective To investigate the effect of Ad IL 2 and Ad IL 3 on immunological reco very. Methods The Ad IL 2 and/or Ad IL 3 were i.p. injected into C57BL/6 mice having received high dose cyclophosphamide (200 mg/kg) 24 hours before. The number and cytotoxicity, Ia antigen expression of peritoneal macrophages, the proliferation and NK cell activity of splenic cells were observed. Results After i.p. injection with Ad IL 3, the number, cytotoxicity and Ia antigen expression of peritoneal macrophagee were increased. After i.p. injection with Ad IL 2, the proliferation and NK cell activity of splenic cells were significantly augmented. As i.p. injection with Ad IL 2 and Ad IL 3 simultaneously, both the peritoneal macrophages and splenic cells were activated. Conclusion Intraperitoneal injection of Ad IL 2 and Ad IL 3 can promote immune recovery after high dose chemotherapy