逆灸对心肌缺血大鼠HSP70的影响

Infection of HSP70 by Preventive Moxibustion on Ischemia Reperfusion Rats

目的:观察逆灸对心肌缺血大鼠HSP70表达的影响,并与缺血预处理的效果进行比较,探讨逆灸有效性和对心肌的保护机制。方法:①选用健康Wistar大鼠30只,雄性,体重(250±20)g,随机分成5组:正常对照组、假手术组、心肌缺血组、心肌缺血预处理组、逆灸组,每组6只。②免疫组织化学法检测大鼠心肌组织HSP70表达。③计量资料差异比较采用SPSS软件统计。结果:逆灸"内关""膻中"穴可明显增加心肌缺血再灌注损伤型大鼠心肌组织中HSP70的表达,与IR组比较差异显著(P<0.05),且与IP组无显著差异(P>0.05)。结论:逆灸与缺血预处理均可增加HSP70的表达,减轻心肌缺血再灌注对心肌造成的损伤,丰富了"治未病"理论。

Objective： To observe the influence of HSP70 by preventive moxibustion on ischemia reperfusion rats, and compare with the effect of Ischemic Precondition, discuss the effectiveness and protection mechanism of preventive moxibustion. Methods： ①Totally 30 healthy male Wistar rats were randomly divided into 5 groups： A control group, a false surgery group, a ischemia reperfusion group, a ischemic precondition group and a preventive moxibustion group.②In munohistochemical test on HSP70 of myocardial tissue of big rats. ③Statistical measurement data of differences comparison was by SPSS. Results ： Preventive moxibustion on Neiguan and Shanzhong can significantly increase the expression HSP70 in the tissue of rats, which varies considerably compared with the IR group （ P 〈 0.05 ） and no significant variation compared with the IP group （ P 〉 0.05 ）. Conclusion ： Preventive moxibustion and Ischemic Precondition can both increase the expression of HSP70, reduce the myocardial injury of Ischemia rechemia . The manipulation enriches the theory of ＂treatment before being sick＂.