应用SSR标记分析大豆种质资源的遗传多样性

Genetic Diversity Analysis of Soybean Germplasm Resources Based on SSR Markers

利用SSR分子标记分析了119个大豆品种的遗传多样性,结果表明:30对SSR引物在119份材料中共检测出159个等位变异,平均每对引物检测到5.30个等位变异;河北省农家品种中平均每对引物检测到5.17个等位变异,育成品种4.87个,省外品种4.93个,表明地方品种的遗传多样性高于育成品种。河北省农家品种、育成品种和省外育成品种依据SSR数据获得的品种间相似系数总体平均值相近,分别为0.698、0.698、0.672,但河北省农家品种较育成品种具有较大的变化幅度。119个品种可被划分为3个类群,在一定程度上能把育成品种和农家品种分开,并反映了一定的品种地域来源。

Genetic diversity of 119 accessions of soybean germplasm resources was evaluated based on SSR molecular markers. The results showed that 159 alleles were detected by using 30 SSR primers pairs with an average of 5.30 in 119 entries, 5.17 in landraces of Hebei, 4.87 in cuhivars and 4.93 in accessions from other provinces. This showed that the genetic diversity of landraces was higher than that of bred-cuhivars. The means of similarity coefficients within each sub-sample, landraces, cuhivars of Hebei and accessions from other provinces, calculated using SSR data were 0. 698, 0. 698, 0. 672, respectively. However, the range of similarity coefficient in landrace samples was wider than that in cuhivar samples of both Hebei and other provinces. 119 entries could be classified into 3 groups. In general, landraces and cuhivars could drop into different groups, and to some degree, a relation existed between the location sources and group partition of the entries.