二巯基乙醇对大鼠骨肉瘤细胞增殖的诱导作用

Effect of 2-mereaptoethanol in the induction of Proliferation of rats' osteosarcoma cells

目的:观察二巯基乙醇(2-ME)对大鼠骨肉瘤细胞增殖的诱导作用。方法:将含10%小牛血清的RPMI1640完全培养基分装成六瓶,每瓶100ml,一瓶不加2-ME作为对照组Ⅰ,其余五瓶分别加入不同剂量2-ME作为实验组,再设一个含10%胎牛血清的完全培养基一瓶作对照组Ⅱ,分别在96孔板中培养大鼠骨肉瘤UMR106细胞,24h、48h和72h后,观察细胞生长状态,采用MTT法检测细胞增殖情况。结果:对照组Ⅰ生长缓慢,对照组Ⅱ生长状态良好,分裂增殖相较多。实验组随着2-ME浓度的增加,细胞生长的速度逐渐增快。其中0.3μl组生长速度适中,细胞生长状态与对照组Ⅱ相似。结论:二巯基乙醇有明显诱导大鼠骨肉瘤细胞增殖的作用。

Objective：To explore the cell proliferation induction of rats＇ osteosarcoma cells treated by different concentration of 2-mereaptoethanol（2-ME）.Methods：Five experimental groups were established and cultured with the RPMI1640 complete medium containing 10% calf serum with different concentration of 2-ME;control groupⅠ,with the RPMI1640 complete medium containing 10% calf serum without 2-ME;and control groupⅡ,with the RPMI1640 complete medium containing 10% fetal calf serum.The culture solutions of different groups were used to culture the rats＇ osteosarcoma UMR106 in 96-hole plate.The cell growth status was observed by microscope.The cell proliferation was detected by MTT assay after treated 24,48 and 72 hours.Results：The cells in the control groupⅠgrew slowly,but the cells in the control groupⅡgrew healthily.In the experimental groups,the cell proliferation increased with the concentration increasing.Conclusion：2-mereaptoethanol plays an important role in the induction of proliferation of rats＇ osteosarcoma cells.