“钾维普”停搏液对未成熟心肌保护作用的研究

Myocardial Protection of Kalium-Verapamil-Propranolol Cardioplegia on Isolated Immature Heart

目的比较"钾维普"停搏液与St.Thomas'Ⅱ停搏液对未成熟大鼠的心肌保护作用。方法离体SD幼大鼠心脏,通过改良Langendorff装置灌流,在未成熟心肌缺血90min再灌注60min期间,实时动态检测心肌张力、心率、收缩力、最大收缩和舒张速度、冠脉流量、复搏时间来评价心功能。结果"钾维普"组心肌收缩力和冠脉流量的恢复优于St.Thomas'Ⅱ组,心率、冠脉流量的恢复接近正常灌流组;再灌注30min后,"钾维普"组心肌收缩力的恢复甚至超过正常灌流组。结论"钾维普"停搏液对未成熟心肌的保护效果优于St.Thomas'Ⅱ停搏液,而且心肌收缩力的恢复优于正常灌流组。

Objective To evaluate the myocardial protective effects of Kalium-Vempamil-Propranolol CKVP） Cardioplesic solution and St.Thomas＇ Ⅱ （STH） Cardioplegic solution on the immature rat myocardium. Methods Sprasue-Duwley immature rots were anesthetized and given heparin. The hearts were perfused by Langendorff perfusion apparatus. Heart rate, tention, contraction force, peak systolic velocity, peak diastole velocity, coronary flow, re-beat time are monitered during the ischemia 90 mln-reperfusion 60 rain. Results The KVP eardioplesia in cardiac contraction force and coronary flow is superior to STH cardioplegia. Compared to control group （continuously perfused 170 min）, in the KVP cardioplesia, cardiac contraction force is stronger after perfusing 30 rain. Conclusions The KVP cardloplesia in protecting the immature rat heart is more effective than STH cardioplesia and superior to control group in cardiac contraction force.