绞股蓝总皂甙提取方法的比较研究

Comparative Study on the Extraction Methods of Total Saponin in Gynostemma

目的:探讨绞股蓝总皂甙最佳的提取方法。方法:首先选用水、80%甲醇、75%乙醇和氯仿等作为溶剂提取绞股蓝总皂甙,从中选择出2种较优试剂,再以回流提取法、索氏提取法、超声波法等提取方法对绞股蓝的总皂甙进行提取,从而选出一种优良的提取方法。并且采用分光光度法测定了绞股蓝总皂甙的含量。结果:研究表明,75%乙醇为绞股蓝总皂甙提取的最佳溶剂,乙醇回流提取法为最优方法。结论:以75%乙醇为提取溶剂的回流提取法是一种高效、简便、节能的绞股蓝总皂甙的提取方法。

Objective： To explore the best extracting methods of total saponin in Gynostemma, Methods： Four solvents, including water, 80 % methanol, 75 % ethanol and chloroform were used to extract the total saponin from Gynostemma and the yield were compared to choose two better solvents. Then backflow extraction, Soxhlet extraction and ultrasonic wave extraction were applied to purify total saponin in Gynostemma to choose the better extraction methods. The content of total saponin was measured by UV- spectrophotometry. Results： The results show that 75 % ethanol was the optimal solvent and backflow extraction with ethanol was the preferably method. Conclusions： Backflow extraction with 75 % ethanol as solvent was a high-effective, convenient and energy-saved method for extracting total saponin in Gynostemma.