鲍曼不动杆菌耐药程度与其主动外排泵蛋白的相关性研究

Study on Correlation between Antibiotic Resistance and Active Efflux Transporters in Acinetobacter Baumannii

目的:探讨鲍曼不动杆菌耐药程度与其主动外排泵蛋白的相关性。方法:首先用纸片扩散法检测64株临床鲍曼不动杆菌对8种抗菌药物的敏感性;将其分为A组(0～2种抗生素耐药)、B组(对3～5种抗生素耐药)和C组(对6～8种抗生素耐药);检测64株临床鲍曼不动杆菌对罗丹明6G的外排情况,筛选出罗丹明6G外排明显增加的菌株;并用逆转录-聚合酶链反应(RT-PCR)方法检测主动外排泵基因AdeABC的表达水平。结果:64株鲍曼不动杆菌中有4株对0～2种抗生素耐药(A组),对3～5种抗生素耐药的有33株(B组),对6～8种抗生素耐药的有27株(C组);多重耐药组鲍曼不动杆菌罗丹6G外排明显增高,外排程度A组<B组<C组;罗丹明6G外排增强的多重耐药不动杆菌主动外排泵基因AdeABC的表达水平显著增高。结论:鲍曼不动杆菌的耐药现象严峻;多重耐药性与药物主动外排增强有关;AdeABC基因的过度表达是鲍曼不动杆菌药物外排增加的重要原因。

Objective： To investigate the function and gene expression of active effiux transporters in Acinetobacter baumannii. Methods： At first, the susceptibilities of 64 strains clinical Acinetobacter baumannii to 8 kinds of antibiotic agents were tested by disc dif- fusion method, then these strains were divided into three groups based on the susceptibilities： group A （resistant to 0-2 antibiotics）, group B （resistant to 3-5 antibiotic） and group C （resistant to 6-8 antibiotics）. The effiux of Rhodamine 6G in these 64 strains of clinical Acinetobacter baumannii was tested and identified, the strains with high increased effiux of Rhodamine 6G were screened out; Reverse transcriptase polymerase chain reaction （RT-PCR） was used to measure the mRNA expression levels of active effiux gene AdeABC. Results： Among the 64 strains, there were 4 strains resistant to 0-2 antibiotics （groupA）, 33 resistant to 3-5 antibiotics （groupB）and 27 resistant to 6-8 antibiotics （groupC）. The effiux of Rhodamine 6G was significantly increased in some multiple resistant strains, and the level ofadeABC were also significantly increased. Conclusion： The antibiotic resistance is very serious in Acinetobacter baumannii. The multiple resistance ofAcinetobacter baumannii is related to the active effiux ability. The overexpression of AdeABC gene induces the in- creased activity of active effiux in Acinetobacter baumannii.