固化液中添加壳聚糖和明胶的磷酸盐骨水泥材料性能测试(英文)

Performance of calcium phosphate bone cement using chitosan and gelatin as well as citric acid as hardening liquid

背景:骨水泥如果固化时间太快,黏度降低,应用时可能会使塑型困难。目的:检测在柠檬酸中添加壳聚糖、明胶配制的固化液与α-磷酸三钙和羟基磷灰石复合的粉剂调和制备骨水泥试样的性能。设计、时间及地点:开放性实验,于2005-03/2006-08在兰州交通大学材料系实验室完成。材料:将α-磷酸三钙粉末与羟基磷灰石粉末混合均匀制得骨水泥粉料,壳聚糖和明胶按不同比例与柠檬酸溶液混合配制的固化液,然后二者调和制得骨水泥。方法:净浆稠度及凝结时间测定仪测定骨水泥的凝固时间,MTS-810型材料试验机测试各种配比骨水泥的压缩强度,扫描电镜观察固化体经37℃生理盐水浸渍2个月后的微观结构。主要观察指标:骨水泥的凝固时间及压缩强度,骨水泥水化反应的pH值及其微观结构。结果:调和液中添加的壳聚糖和明胶,使其黏度明显增加,固化时间延长,试样抗水冲性能提高,样品塑型容易操作,但试样抗压强度有所降低。骨水泥水化反应的pH值随着水化反应的进行逐渐上升,24h时接近生理盐水的pH值。壳聚糖-明胶含量不同可以得到不同机械强度的α-磷酸三钙/羟基磷灰石两相骨水泥,骨水泥试样24h基本达到最大强度,在48h后强度几乎不再变化。结论:固化液中添加壳聚糖、明胶制备的α-磷酸三钙/羟基磷灰石两相骨水泥克服了陶瓷型羟基磷灰石烧结形成、修整困难等缺点,具有塑型容易、使用方便、固化时放热小等优点。

BACKGROUND： When bone cement solidifies fast, the bone cement consistency will be decreased, resulting in difficulty in molding. OBJECTIVE： To test the performance of bone cement prepared with compound power containing α -tricalcium phosphate （ α -TCP） and hydroxylapatite （HA） and hardening liquid containing chitosan and citric acid as well as gelatin. DESIGN, TIME AND SETTING： Open experiment, performed in the Laboratory of Department of Materials, Lanzhou Jiaotong University between March 2005 and August 2006. MATERIALS： α -TCP and HA were evenly mixed to prepare bone cement power. Different proportions of chitosan and gelatin were mixed with citric acid to produce hardening liquid. Then bone cement power and hardening liquid were mixed to form a paste, i.e., bone cement. METHODS： Setting time of bone cement was determined using a Vicat apparatus. The compressive strength of bone cement at different proportions was tested using MTS-810 material tester. After 2 months of physiological saline soaking at 37 ℃, the microstructure of solidified bone cement was observed using scanning electron microscope. MAIN OUTCOME MEASURES： Setting time and compressive strength of bone cement, pH value of hydrated bone cement and the microstructure. RESULTS： After adding chitosan and gelatin in the hardening liquid, the consistency of the concoction obviously increased, the setting time became a little longer and the water-resistance of samples was enhanced, therefore the samples could be easily molded. But the compressive strength of the bone cement decreased a little. The pH value of the hydrated bone cement increased gradually with time and was close to the pH value of physiological saline at hour 24. The compressive strength of the α -TCP/HA bone cement sample had changed with the content of chitosan and gelatin. The maximum strength of the bone cement was achieved at 24 hours and it almost did not change after 48 hours. CONCLUSION： α -TCP/HA bone cement prepared by adding chitosan and gelatin into the hardening liquid is free from the drawback of ceramic HA, including sintering and difficulties in shaping. It has the characteristics of simple-producing, easy application and low heat energy.