HPLC指纹图谱法鉴别远志及其伪品

HPLC fingerprint spectra for discriminating spurious breed of Polygala radix

目的建立远志药材的HPLC指纹图谱,用以鉴别远志与其伪品。方法采用高效液相法,色谱柱为Hypersil C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.5%磷酸(25∶75),流速1.0mL/min,检测波长310 nm。结果建立了正品远志药材的对照指纹图谱,标示了共有指纹峰,可用于区别远志伪品。远志HPLC指纹图谱与其伪品的液相色谱图存在明显的差异。结论方法简便、特异,可用于远志药材、饮片以及粉末的鉴别,具有较高的实用价值。

Aim To establish the HPLC fingerprint spectra for discriminating spurious breed of Polygala radix. Methods Polygala radix was detected by HPLC method with the conditions of Hypersil C18 chromatographic column （4. 6 mm × 250 mm, 5 μm）, acetonitrile-0. 5 % H3 PO4 （25： 75 ） as mobile phase, flow rate of 1.0 mL/min and 310 nm detection wavelength. Results The HPLC fingerprint spectra of Polygala radix with labled common characteristic peaks was established. It could be used for distinguishing spurious breed of Polygala radix. Conclusion The method possesses practical value with simple, sensitive and specific features for discriminating Polygala radix＇s medical material, decoction pieces and powder.