摘要
目的从泡菜汁中分离产凝集素乳杆菌并鉴定。方法通过凝集实验筛选产凝集素的乳杆菌,用聚合酶链式反应(PCR)扩增16S rDNA可变区基因鉴定产凝集素乳杆菌。结果此菌株呈革兰阳性,短杆状,产乳酸。通过同源性比较,此菌株与Lactobacillus plantarum strain p215的同源性为99%。结论分离得到了产凝集素的菌株,鉴定表明此菌株为植物乳杆菌。
Objective To isolate and identify lectin-producing lactobacillus from sauerkraut juice. Methods The lectin-producing lactobacillus was isolated by hemagglutination assay and identified by polymerase chain reaction (PCR) amplification of variable regions of the 16S rDNA gene. Results This strain was gram-positive, short bacilliform and lactic acid-producing. The homology to Lactobacillus plantarum strain p215 was 99 %. Conclusion The lectin-producing lactobacillus is obtained and identified as Lactobacillus plantarum.
出处
《食品与药品》
CAS
2008年第5期9-11,共3页
Food and Drug
基金
国家自然科学基金资助项目(30760304)
云南省科学研究基金资助项目(2006C0046M)
关键词
泡菜
乳杆菌
分离
鉴定
凝集活性
DNA
核糖体
序列分析
sauerkraut
lactobacillus
isolation
identification
agglutinating activity
DNA
ribosome
sequence analysis