甲基丙烯酸基质的毛细管电色谱整体柱的制备与应用

Preparation of Methacrylic Acid-based Monolithic Column for Capillary Electrochromatography by Photopolymerization

以甲基丙烯酸(MAA)为功能单体,同时又作电渗流改性剂,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,甲苯和异辛烷为致孔剂,Irgacure1800为光引发剂,采用紫外光引发原位聚合反应制备出毛细管电色谱整体柱。对影响电渗流的因素如pH、乙腈含量及离子强度等进行了讨论。使用制备的整体柱在3种模式(加压电色谱、气压驱动和电色谱)下对7种中性物质(硫脲、苯、甲苯、乙苯、萘、联苯和菲)的混合物实现基线分离,同时还可实现酸性物质(邻羟基苯甲酸、苯甲酸、苯乳酸、扁桃酸)和碱性物质(苯胺、甲苯胺、乙酰基苯胺和N-甲基苯胺)的快速分离。

A monolithic polymer was prepared by in-situ photopolymerization, using methacrylic acid （MAA） as a functional monomer, ethylene glycol dimethacrylate （EDMA） as a cross-linking agent, toluene and isooctane as porogenic solvents and Irgacure 1800 as an initiator. The effects of the content of acetonitrile, pH values, and salt concentration of buffers on electroosmotic flow （EOF） were investigated. The results showed that baseline separation of some neutral compounds was achieved on this monolithic column in the modes of pressure-driven capillary electrochromatography （p-CEC） , capillary electrochromatography （ CEC ） and low- pressure driven （LPLC）. Meanwhile, some acidic or basic compounds can be separated on this monolithic column under relatively mild conditions.