酶联免疫吸附法测定血浆中芍药苷浓度

Enzyme-linked Immunosorbent Assay for the Determination of Paeoniflorin in Plasma

建立了间接竞争酶联免疫吸附法测定血浆中芍药苷浓度。在10～200μg/L浓度范围内,线性关系良好,线性回归方程为y=-0.1721logC+1.9645,相关系数0.9927;回收率为89%～102%。运用本方法测定了大鼠一次灌胃后血浆中的芍药苷浓度。比较了分别给药芍药苷单体及中药复方四逆散提取物后,血浆中的芍药苷浓度。研究结果表明:四逆散配伍组方后,芍药苷的生物利用度较低。

A method was developed for the determination of paeoniflorin in plasma by competitive enzyme- linked immunosorbent assay（ELISA）. The artifical antigen was used as immunogen and applied in New Zeal- and rabbits to get the anti-paeoniflorin antibodies. Hapten coupled with OVA was used as coating antigen. The linear concentrations ranged from 10 μg/L to 200 μg/L and the calibration curve was Y = -0. 1721 logC ＋ 1. 9645. Recoveries for the standard addition paeoniflorin to blank palsma were 89% - 102%. The method has been successfully applied to determine the concentration of paeoniflorin in rat plasma. After an oral administra- tion of the Si-Ni-San decoction, the bioavailability of paeoniflorin was lower than a single dose of paeoniflorin.