神府煤生物转化用溶煤菌株的优选研究

Study on Screening of Strains for Bioconversion of Shenfu Coal

为提高神府煤的生物转化率和产物的定向转化选择性,在固体无机盐培养基中,以神府风化煤为唯一碳源,采用平板划线法从分离自神府煤矿洗煤废水的47株菌中筛选出了3种溶煤效果较好的菌株XK-a、XK-b、XK-c。分别用这3种菌株在液体无机培养基中转化神府原煤和经光氧化6 h后的煤,结果发现XK-b具有较强的溶煤能力,并且对光氧化神府煤有更强的转化能力,光氧化煤的生物转化率最高达到22.7%,水溶性产物经NaOH溶液调pH值后碱沉淀物产率可达17.5%。光氧化煤经XK-b转化前后的FTIR分析表明,光氧化煤和XK-b转化残煤的光谱特征非常相似,但其经XK-b转化产生的水溶性物质的醚键结构与光氧化煤有明显差别,羟基官能团明显增加。

To increase Shenfu coal bioconversion rate and selectivity of its bioconversion products,3 strains, XK-a,XK-b,XK-c,were screened from 47 strains isolated from Shenfu coal-washing waste water,by scratching method on flat plate in solid inorganic medium with Shenfu weathered coal as solely carbon source. In liquid inorganic medium,their abilities to convert Shenfu raw coal and 6 h photooxidized coal into liquid were tested. It was found that XK-b had higher ability for conversion of Shenfu coal,and its maximum conversion rate for 6 h photooxidized coal arrived to 22.7%. Its water-soluble products were precipitated by NaOH and the precipitate yield was 17. 5%. 6 h Photooxidized coal and its bioconversion products by XK-b were characterized by FTIR analysis. The spectral characteristics of the residue were very similar with those of 6 h photooxidized coal. But the water-soluble products had much more hydroxyl groups, and the special ether bond structure greatly differed from that of photooxidized coal.