摘要
建立了一种用于检测土壤中乙草胺残留量的间接竞争酶联免疫分析(EUSA)方法。结果表明,该方法的最低检测限为0.1μg·L^-1,线性检测范围为0.2~10μg·L^-1,土壤样品中乙草胺添加水平在5~50μg·kg^-1范围内的回收率在81.20%~93.27%之间,批次内、批次间变异系数均低于7.50%。经高效液相色谱(HPLC)确证,其准确度、精密度都符合残留分析的要求。
The method of indirect ELISA for monitoring acetochlor residue in soil has been established successfully. The limit of detection is 0. 1 μg · L^-1 ,and the standard curve is linear with the acetochlor concentration from 0.2 μg · L^-1 to 10μg · L^-1. The recoveries of analytes at the fortified levels from 5 μg · kg^-1 to 50μg · kg^-1 range from 81.20% to 93.27% ,and the coefficients of variation for distribution and the coefficients of variation for precession are both lower than 7.50% ,which satisfy the requiremerit of residual analysis with the proof of HPLC.
出处
《化学与生物工程》
CAS
2008年第9期72-74,共3页
Chemistry & Bioengineering
基金
湖北省重点科技攻关资助项目(2005AA101C33)