孕妇人巨细胞病毒-PP65及IgM的检测分析

Detections of HCMV-PP65 and HCMV-IgM for pregnant women

目的探讨妊娠妇女人巨细胞病毒的感染状况及监测效果。方法分别采用酶联免疫吸附试验和间接荧光免疫染色法检测2004年1月～2007年6月在深圳市福田区第二人民医院行产前检查的760例孕妇外周血人巨细胞病毒-PP65抗原血症和人巨细胞病毒-IgM。结果孕妇人巨细胞病毒感染阳性率为4．87％，其中人巨细胞病毒-IgM抗体阳性率为1．58％（6／380），外周血白细胞人巨细胞病毒-PP65抗原血症的检测结果阳性率为8．16％（31／380）。两种检测方法比较χ^2=18．99，P〈0.001，有非常显著性差异。结论同时应用人巨细胞病毒-IgM抗体和人巨细胞病毒、PP65抗原检测方法可对孕妇妊娠早期人巨细胞病毒活动性感染进行监测及治疗指导。

Objective To investigate infection status of human cytomegalovirus （HCMV） in pregnant women and analyze the detection result. Methods HCMV-PP65 antigenemia in peripheral blood leukocytes of 760 pregnant women who received prenatal examination in The Second Futian District People＇ s Hospital from January, 2004 to June, 2007 was detected by using indirect fluorescent immune staining method. At the same time, HCMV-IgM of the pregnant women was tested by ELISA. Results Among pregnant women,the positive infection rate of HCMV was 4.87%, of which, HCMV-IgM positive rate was 1.58% （6/380） and the positive detectable rate of HCMV-PP65 antigenemia in peripheral blood leukocytes of pregnantwomen was 8.16% （31/380）. In HCMV positive detectable rate, there was very significant difference between the two detecting methods （χ^2 = 18.99, P 〈 0.01 ）. Conclusion Combined detections of HCMV-PP65 anfigenemia in peripheral blood leukocytes and serum HCMV-IgM can monitor active infection of HCMV in the early stage of pregnancy and direct the antiviral treatment.