COL1a1、COL3a1在发育性髋脱位患儿圆韧带的表达

Expression of COLlal and COL3al in the ligamentum teres of developmental dislocation of the hip

[目的]探求发育性髋脱位(developmental dislocation of the hip,DDH)患儿与正常儿圆韧带中Ⅰ、Ⅲ型胶原在mRNA及蛋白水平的表达差异。[方法]选取性别相同年龄相近的6对发育性髋脱位患者及正常儿配对比较。采用半定量RT-PCR及Western-Blot法检测COL1a1、COL3a1在mRNA及蛋白水平的表达。图像分析软件进行量化分析,并经统计学处理。[结果]COL1a1在DDH组mRNA及蛋白水平表达均较正常对照组降低(P<0.01);COL3a1在DDH组mRNA及蛋白水平表达与正常对照组无显著差异(P>0.05)。[结论]DDH患儿圆韧带中Ⅰ型胶原在mRNA及蛋白水平表达较正常同性同龄儿降低,可能是导致DDH患儿髋关节松弛的原因。DDH患儿髋关节松弛可能与Ⅲ型胶原含量无关。

[ Objective] To investigate the expression of collagen type Ⅰ and Ⅲ at mRNA and protein level in the ligamentum teres of developmental dislocation of the hip （DDH） and the normal hip. [Method] There were 6 pairs joint laxity of patients of DDH group and normal control group with paired control of same sex and age. Semiquantity RT - PCR method was used to detect the COL1a1 and COL3al in the ligamentum teres at mRNA level. Western -Blot method was used to detect the COL1a1 and COL3a1 in the ligamentum teres at protein level. The quantitative analysis of the COL1a1 and COL3a1 were performed by professional image software and the results were analyzed with standard statistical methods. [ Result] At both mRNA and protein level COL1a1 expression were decreased in the DDH group compared to the control group （P 〈 0. 01 ） . There was no significant difference for COL3a1 between DDH and control group at either mRNA or protein level （P 〉 0. 05 ） . [ Conclusion] The decreased collagen Ⅰ expression at mRNA and protein level in the ligamentum teres of the children with DDH may lead to hip joint laxity. Hip joint laxity in DDH may be independent to the content of collagen Ⅲ.