红霉素和阿伦磷酸钠防治人工关节松动动物实验研究

Erythromycin and alendronate inhibit aseptic loosening in murine osteolysis model

[目的]应用植骨气囊模型观察红霉素和阿伦磷酸钠防治人工关节松动的效果。[方法]应用8～10周大的BALB／c小鼠建立气囊模型，气囊成熟后在气囊内植入同基因小鼠颅骨，同时在气囊内注入超高分子聚乙烯颗粒。实验分4组：空白组气囊和腹腔均注射生理盐水（阴性对照）；颗粒刺激组气囊注射聚乙烯颗粒，腹腔注射生理盐水（阳性对照）；红霉素组气囊注射聚乙烯颗粒，腹腔注射红霉素；阿伦磷酸钠组气囊注射聚乙烯颗粒，腹腔注射阿伦磷酸钠。14d后处死，取囊壁和植入颅骨组织进行组织形态学和分子生物学检测。[结果]红霉素和阿伦磷酸钠组破骨细胞激活均受抑制，两者无显著差别。但红霉素组IL-1、TNF和VEGF含量明显下降，气囊炎性反应减轻，而阿伦磷酸钠无此抗炎作用。[结论]本试验证明红霉素和阿伦磷酸钠均有可能成为延缓和治疗人工关节松动的药物。

[ Objective] To observe whether erythromycin and alendronate are effective in treating aseptic loosening in a murine osteolysis model. [ Method] Air pouches were established in the back of BALB/c mice by injecting sterile air subcutaneously. After the air pouches were mature, grafts of calvaria from syngeneic mouse donors were implanted in the air pouches and polyethylene debris was injected into the air pouches. Mice were divided into 4 groups. In the first group, saline was injected into the air pouches. In the other three groups, polyethylene debris was used. Saline, erythromycin and alendronate were injected intraperitoneally separately. Pouch tissues were collected at 14 days after polyethylene debris administration for molecular and his- tologic analysis. [ Result] Both erythromycin and alendronate inhibited osteoclastogenesis and bone resorption, but alendronate was not as effective as erythromycin in anti - inflammation. [ Conclusion ] Both erythromycin and alendronate can be promising drugs for the treatment of aseptic loosening.