斑蝥黄质对D-半乳糖老龄大鼠骨质影响

Influence of canthaxanthin on D-galactose induced osseous changes of rat

目的:研究斑蝥黄质对D半乳糖致衰老大鼠骨质的影响。方法:将45只6周龄Wistar雄性大鼠随机分为3组(衰老模型组、斑蝥黄质组、青年对照组),每组15只,另设16月龄Wistar雄性大鼠15只作为老龄对照组。其中用D半乳糖对衰老模型组、斑蝥黄质组大鼠进行连续5个月皮下注射(每日1次,每次20mg/kg);5个月后连同青年对照组和16月龄老龄对照组一起进行骨常规参数测定并比较组间差异。结果:衰老模型组骨密度,骨结构力学参数,生物力学参数,骨钙、镁、锰及羟脯氨酸含量均比青年对照组有显著降低(P<0.01),而骨磷含量、骨及血清碱性磷酸转移酶均比青年对照组有显著升高(P<0.01),且这些变化与老龄对照组的变化趋势一致。斑蝥黄质组生物力学参数,骨钙、镁、锰及羟脯氨酸含量,骨磷含量、骨及血清碱性磷酸转移酶等指标均与衰老模型组呈现显著差异(P<0.01)。结论:长期大量摄入D半乳糖导致大鼠衰老同时可能诱发其发生骨质变化,而斑蝥黄质可以预防或降低此类致老性骨质变化的发生。

Objective：To study the influence of canthaxanthin on D-galactose induced osseous changes of rat. Methods： Forty-five six-week-old Wistar male rats were randomly divided into model group,canthaxanthin group and young control group. In addition,15 sixteen-month-old Wistar male rats were used as old control group. Model group and canthaxanthin group were given injections of D-galactose for 5 months（20 mg/kg/once per-day） to cause aging of rat. Then routine osseous parameters were tested and compared among the 4 groups. Results：Compared with young control group,the BMD,parameters of structural mechanics and biomechanics,bone calcium,manganese,magnesium and the content of hydroxyproline in the model group decreased significantly（P〈0.01）,however,the content of bone phosphorus,the activity of bone and serum ALP increased significantly （P〈0.01）. Those changes of the model group were the same as the old control group,but the changes in the canthaxanthin group significantly differed with the model group （P〈0.01）. Conclusion：The high does of D-galactose intake can cause aging and osteoporosis at the same time in rat,but canthaxanthin can prevent and inhibit D-galactose induced osseous changes.