BDNF基因多态性与女性抑郁症帕罗西汀治疗反应的关系

Brain-derived neurotrophic factor gene polymorphism(G196A) and paroxetine response in female depressive disorder

目的：探讨脑源性神经营养因子（brain derived neurotrophic factor，BDNF）基因G196A多态性与女性抑郁症患者帕罗西汀治疗反应的相关性。方法：检测63例女性抑郁症患者（患者组）和113例健康对照者（对照组）汉密尔顿抑郁量表评分（HAMD）和BDNF基因G196A的基因型，患者组治疗4周后复测HAMD，并根据患者对帕罗西汀的治疗反应分为反应组（Rp组，47例）和非反应组（non-Rp组，16例）。结果：患者组与对照组的基因型、等位基因频率和等位基因携带分布无显著差异。BDNF基因G196A多态性的基因型、等位基因频率及等位基因携带Rp组和non-Rp组有显著差异。Rp组A等位基因携带（GA＋AA）显著高于non-Rp组（χ^2=6，336，P=0．012，OR=5．429，95％CI=1，593～18．502），两组A等位基因频率分布也有显著差异（χ^2=4．608，P=0，0318，OR=2，500，95％CI=1．068～5．850）。结论：BDNF基因G196A多态性与女性抑郁症患者帕罗西汀治疗反应相关，A等住基因携带者对帕罗西汀的反应较好。

Objective： To explore the relationship between the G196A polymorphism in the brain-derived neurotrophic factor（BNDF） gene and the response to paroxetine in a Chinese female population with depressive disorder （ DD ）. Methods： Polymerase chain reaction （PCR） was used to identify the genotype of 113 healthy controls and 63 DD patients. The amplified DNA was digested with the restriction endonuclease BbrP Ⅰ and the product was electrophoresed in 5% polyacrylamide gel electmphoresis （PAGE） and stained with silver. 2A-item Hamilton Depression Rating （HAMD） scale for the appraisal of depression degree was used. 63 patients were divided into responders （ Rp ,47 ） and nonresponders （ Non-Rp, 16）. Results ：The genotype, allele, and al- lele-carrier distributions did not differ significantly between DD patients and normal controls. The genotype, allele, and allele-carrier distributions for the G196A polymorphism differed significantly between Rp and Non-Rp. The frequency of A-allele carriers （ GA ＋ AA） was higher in Rp than that in Non-Rp （χ^2= 6.336 ,P = 0.012, OR = 5.429,95% CI = 1. 593-18.502）, as was the A-al- lele frequency （ χ^2 = 4. 608, P = 0.0318, OR = 2. 500,95 % CI = 1. 068-5. 850 ）. Conclusion： The G196A polymorphism of BDNF is associated with paroxetine efficacy in female depressive disorder,with A-allele carriers responding better to paroxetine treatment.