sIL-15Rα与脾细胞孵育后对小鼠黑色素瘤细胞生长的免疫调节作用

Immunomodulatory effects of spleenic cells added by sIL-15Ralpha on the regulation of melanoma cell growth

目的:研究可溶性IL-15Rα与脾细胞共同孵育后对小鼠黑色素瘤细胞生长的免疫调节作用。方法:制备小鼠脾细胞,分成两组,一组加入可溶性重组IL-15Rα(sIL-15Rα),另外一组不加,培养48 h后,分离两组的贴壁细胞和非贴壁细胞,流式细胞术(FCM)分析CD4、CD8、B220、CD11c、CD1a的表达;并把上述4组细胞(即脾细胞非贴壁细胞组,脾细胞贴壁细胞组,脾细胞+sIL-15Rα非贴壁细胞组,脾细胞+sIL-15Rα贴壁细胞组)和黑色素瘤细胞一起分别注射到小鼠腹部皮下,观察小鼠腹部皮下肿瘤生长抑制情况,对照组只注射小鼠黑色素瘤细胞。结果:脾细胞+sIL-15Rα贴壁细胞组小鼠黑色素瘤的生长速度显著小于其他各组;FCM分析,此组细胞主要成分可能为树突状细胞(DC)。结论:sIL-15Rα与脾细胞共同孵育后,可能通过DC的作用,对黑色素瘤的生长进行免疫调节,从而抑制瘤细胞的生长。

AIM： To study the immunomodulatory effects of spleenic cells added by souble IL-15Ralpha on the regulation of melanoma cell growth. METHODS： The spleenic cells of mice were prepared for culture for 48 hours by adding or not adding soluble IL-15Ralpha （sIL- 15Ralpha）. Then the adherent cells and non- adherent cells were separated. FACS was used to detect the expression of CD4, CD8, B220, CD11c and CD1a; as mentioned above, the four groups （non-adherent cells of spleenic cells, adherent cells of spleenic cells, non-adherent cells of spleenic cells added by sIL-15Ralpha, adherent cells of spleenic cells added by sIL-15Ralpha） were mixed with melanoma cells separately, then injected intraperitoneally into the cells to mice to observe the tumor volume of the mice, control group was only injected intraperitoneally into melanoma cells. RESULTS： The melanoma growth rate of mice with adherent cells added by sIL-15Ralpha was significantly less than that of the other groups. FACS showed the molecules of this group were possibly dendritic cells. CONCLUSION： sIL- 15Ralpha added by spleenic cells can make an immunomodulatory effets possibly mainly by the effects of dendritic cells and inhibit the growth of tumor cells.