草木犀石油醚提取物对NF-κB和血红素氧合酶1表达的影响

Effect of petroleum ether extract from Melilotus suaveolens Ledeb on the expression of NF-κB and Heme oxygenase 1

目的:探讨草木犀的石油醚提取物在炎症中的作用。方法:通过LPS干预RAW264.7细胞系建立炎症细胞模型,免疫细胞化学法检验NF-κB的表达及分布变化,实时荧光定量RT-PCR法检测血红素氧合酶1(HO-1)的基因表达,Western blot法测定HO-1的蛋白表达量。结果:免疫细胞化学法结果显示药物干预时胞质被染为棕色,NF-κB多分布在胞质未被激活;仅有LPS干预时,胞核被染为棕色,NF-κB集中分布在细胞核表达增强。药物提取物干预后HO-1的mRNA表达水平明显升高,且呈剂量依赖型关系;Western blot结果显示药物干预后HO-1的蛋白表达水平也明显升高。结论:草木犀的石油醚提取物通过抑制NF-κB的激活,同时促进HO-1的释放而发挥一定的抗炎作用。

AIM： To study the anti-inflammatory effect of petroleum ether extract from Melilotus suaveolens Ledeb. METHODS： Inflammatory cell model was constructed by LPS acting on the RAW264.7 cell line. The expression and distribution of NF-κB were detected using immunocytochemical method. The expression of mRNA and protein of Heme oxygenase 1 （HO-1） were detected by real-time PCR and Western blot. RESULTS： The immunocytochemical analysis showed that the cytoplasm stained to brown presented NF-κB inactivation after the intervention of petroleum ether extract while the cell nucleus stained to brown presented NF-κB activation after the only intervention of LPS. The expression of HO-1 mRNA was significantly enhanced by the extract in a dose-dependent manner, and the expression of HO-1 protein was markedly enhanced too. CONCLUSION： The petroleum ether extract from Melilotus suaveolens Ledeb can resist inflammation by inhibiting the activation of proinflammatory factor NF-κB and enhancing the expression of anti-inflammatory factor HO-1.