亚洲带绦虫烯醇酶基因及其蛋白质的结构与功能

Structure and Characteristics of Gene and Protein of Enolase from Taenia asiatica

【目的】分析和预测亚洲带绦虫烯醇酶基因及其编码蛋白的结构和特性,用于指导其生物学功能的实验研究。【方法】利用美国国家生物技术信息中心和瑞士生物信息学研究所的蛋白分析专家系统中有关基因和蛋白的序列和结构信息分析的各种工具,结合其他生物信息学分析软件包,如Pcgene和Vector NTIsuite,从亚洲带绦虫全长cDNA质粒文库中识别烯醇酶基因及其编码区,分析、预测该基因编码的蛋白质的理化特性、翻译后的修饰位点、功能域、亚细胞定位、拓扑结构、二级结构、三维空间构象等。【结果】该基因全长1737bp,编码区为第205～1503bp,编码433个氨基酸,为全长基因。GenBank中与棘口吸虫烯醇酶氨基酸序列同源性最高,达78%。相对分子量理论预测值为46653.5 Ku。没有质体、线粒体定位序列。预测编码蛋白有1个跨膜区,3个亲水性部位。与吸虫属的烯醇酶进化关系最近。【结论】应用生物信息方法从亚洲带绦虫成虫cDNA文库中筛选出了亚洲带绦虫烯醇酶cDNA全长序列并预测得到其结构与功能方面信息。

[Objective] To get the messages on the structures and characteristics of enolase from Taenia asiatica （T.a.ENO） by bioinformatics. [Methods] A full-length cDNA sequence encoding enolase from cDNA plasmid library of Taenia asiatica was identified by using tools of bioinformatics at webs sites of NCBI. The characteristics of the deduced protein including the physico-chemical characteristics, modification sites after translation, domains, subcelluar location, topological structure, secondary structures, and 3D structure were predicted by employing bioinformatics software package supplied by the website of ExPaSy. [Results] The full cDNA sequence encoding T.a.ENO includes a complete open reading frame of 1299bp which encoded a putative protein of 433 amino acids. The coding region is 205 bp - 1503 bp. The amino acids sequence has a high identity with enolase from other species in GenBank. The protein has one transmembrane region and stable physico-chemical characteristics. The molecular weight of T.a.ENO is predicted to be 46653.5u. The protein has three hydrophilic regions. The relationship of phylogenesis between T.a.ENO and enolase of other trematodes is close. [Conclusion] The cDNA sequence encoding enolase was screened from cDNA library of adult Taenia asiatica by bioinformatics. The structure and characteristics of the gene and protein of T.a.ENO were obtained.