促红细胞生成素对颅脑损伤后线粒体ATP酶活性及自由基代谢的影响

Effect of recombinant human erythropoietin on ATPase activity and free radical metabolism in the neuronal mitochondria after brain injury in rats

目的探讨重组促红细胞生成素（rhEPO）对大鼠颅脑损伤后脑组织线粒体ATP酶、超氧化物歧化酶（SOD）的活性及丙二醛（MDA）水平的影响。方法建立大鼠自由落体脑挫裂伤模型，伤后立即腹腔注射rhEPO，采用生化检测的方法分别测定治疗后6h、12h、24h和48h及各自损伤对照组大鼠脑组织线粒体ATP酶和SOD的活性以及MDA水平。结果rhEPO治疗后12h、24h和48h大鼠脯组织线粒体ATP酶和SOD活性均明显高于各自时间点损伤对照组．而MDA水平则明显低于各自时间点损伤对照组，差异均有统计学意义（P〈0．05）。结论rhEPO可以通过影响线粒体功能而减轻大鼠创伤性脑损伤后的继发性脑损害，从而改善预后。

Objective To investigate the effect of recombinant human erythropoietin （rhEPO） on the activity of ATPase and superoxide dismutase （SOD） and malondialdehyde （MDA） content in the mitochondria of rat neurons after brain injury. Methods rhEPO was intraperitoneally injected in rats immediately after brain contusion injury induced by impact of a free-falling object. In the control rats, an equivalent volume of saline was injected intraperitoneally after the brain injury. At 6, 12, 24, and 48 h after rhEPO or saline injection, the mitochondria were isolated from the brain neurons of the rats to determine the activity of ATPase and SOD and the content of MDA. Results At 12, 24 and 48 h after rhEPO treatment, the activity of ATPase and SOD increased and MDA content decreased significantly in the mitochondria of the brain neurons as compared with the measurements in the control group at the corresponding time points （P〈0.05）. Conclusion rhEPO treatment can ameliorate secondary brain injury in rats following brain trauma by modulating the mitochondrial function.