HPLC测定香砂养胃丸中橙皮苷和柚皮苷含量

Determination of Hesperidin and naringoside in xiangsha yangwei pill by HPLC

Hesperidin and naringoside in xiangsha yangwei pill has been determined by the HPLC,in which the DiamonsilTMC18(4.6 mm×150 mm,5 μm)was used as the column at UV 283 nm and the CH3CN-H2O solution(22∶78) was made use of mobile phase,in which H3PO4 was used as agent to regulate pH(3.5),the flow rate is 1.2 mL·min-1,there was a fine separated degree between the pending component and other component and the lineal range of Hesperidin and naringoside is 0.25μg^1.49 μg and 0.023μg^0.14 μg respectively.The average recoveries of Hesperidin and Naringoside were 99.3% and 102.6% respectively with RSD being 1.2% and 2.5%.The result can give a simple,effective method for controlling the quality of xiangsha yangwei pill.

Hesperidin and naringoside in xiangsha yangwei piU has been determined by the HPLC, in which the DiamonsilTMCis （4. 6 nun × 150 mm,5 μm）was used as the column at UV 283 run and the CH3CN-H2O solution （22：78） was made use of mobile phase,in which H3PO4 was used as agent to regulate pH（3.5 ） ,the flow rate is 1.2 mL · min^-1 ,there was a fine separated degree between the pending omponent and other component and the lineal range of Hesperidin and naringoside is 0. 25μg - 1.49μg and 0. 023μg -0. 14μg respectivdy. The average recoveries of Heaperidin and Naringoside were 99. 3% and 102. 6% respectively with RSD being 1.2% and 2.5% . The result can give a simple,effective method for controlling the quality of xiangsha yangwei pill.