PCR方法检测美人蕉黄斑驳病毒

Detection of Canna yellow mottle virus by PCR

美人蕉黄斑驳病毒病是危害美入蕉的重要病害之一，我国目前尚无该病发生的报道。本文以健康和感病植株为材料，进行总DNA的提取和PCR扩增，从感病植株中扩增出与目的片段大小相符的产物，而健康植株中则未扩增出。PCR扩增产物经克隆、测序后证实，与已报道的美入蕉黄斑驳病毒（Canna yellow mottle virus，CaYMV）序列同源性高达97％～99％。经优化反应条件，建立了稳定的美入蕉黄斑驳病毒PCR检测方法。

The yellow mottle disease caused by Canna yellow mottle virus （CaYMV） is a major disease in canna, it has not been reported in China. DNA extracted from healthy and infected canna was amplified by PCR. The resuits showed that the target fragment could be amplified from the infected sample while no amplified products were obtained from the healthy sample. Cloning and sequencing analysis indicated that nucleotide sequence of the target fragment was 97% - 99% identical to the reported CaYMV genomic sequence. A reliable PCR assay was established to detect CaYMV through optimizing the reaction condition, and it is suitable for rapid quarantine detection.