抗线粒体抗体M2亚型抗体三种检测方法的比较及其对原发性胆汁性肝硬化的诊断价值研究

Compared with three methods to detect M2 autoantibodies and evaluation of measurement in diagnosis of primary billiary cirrhosis

目的比较三种方法检测抗线粒体抗体M2亚型(AMA-M2)抗体在原发性胆汁性肝硬化诊断中的应用价值。方法分别用以丙酮酸脱氢酶复合体(PDC)为靶抗原的ELISA法;以三联体(BPO)为靶抗原的ELISA法;以天然M2抗原和BPO融合蛋白M2-3E(BPO)为靶抗原的ELISA法检测原发性胆汁性肝硬化患者,自身免疫性疾病患者和健康体检者血清中的AMA-M2抗体。结果以PDC为靶抗原的ELISA法测定AMA-M2抗体检出率(敏感性)达81.25%,特异性达97.15%;以BPO为靶抗原的ELISA法测定AMA-M2抗体检出率(敏感性)达88.54%,特异性达98.37%;以M2-3E(BPO)为靶抗原的ELISA法测定AMA-M2抗体检出率(敏感性)达96.88%,特异性达97.15%。结论包被有天然M2抗原和BPO融合蛋白这两种抗原联合制备的三联体检测AMA-M2抗体的阳性率最高,特异性达到97.15%,为原发性胆汁性肝硬化的诊断提供了简便、快速而有效的手段。

Objective To compare three measurement of M2 autoantibodies in diagnosis of primary biliary cirrhosis（PBC）. Methods Serum from 96 patients with primiary biliary cirrhosis,a control panel of 100 patients with other autoimmune disease and 50 healthy blood donors were analysed using the Anti-M2 ELISA,Anti-BPO ELISA,Anti-M2-3E ELISA.Results The sensitivity of theAnti-M2 ELISA for PBC was 81.25%, with a specificity of 97.15%;The sensitivity of the Anti-BPO ELISA for PBC was 88.54% ,with a specificity of 98.37% ;Thesensitivity of the Anti-M2-3E（BPO） ELISA for PBC was 96.88%, with a specificity of 97.15%. Conclusion The sensitivity of the Anti-M2-3E（BPO）ELISA for PBC was best,with a high specificity,It should be used as a powerful and specific maker for the diagnosis of PBC.