Lactacystin对体外C6胶质瘤细胞超微结构影响的研究

Study on the subcellular structure's alteration of C6 glioma cell in vitro after treated with proteasome inhibitor Lactacystin

目的探讨蛋白酶体抑制剂Lactacystin对体外C6胶质瘤细胞超微结构的影响。方法Lactacystin处理体外培养的C6胶质瘤细胞,HE染色后光镜观察细胞的死亡情况,锇铀铅染色后透射电镜观察细胞超微结构变化。结果Lactacystin处理体外C6胶质瘤细胞24 h后,光镜下见胞浆浓缩深染,胞核染色质致密浓缩,透射电镜下见细胞表面微绒毛消失,核皱缩,染色质致密浓缩、边集。结论蛋白酶体抑制剂Lactacystin可以诱导体外C6胶质瘤细胞凋亡。

Objective To investigate the influence of proteasome inhibitor Lactacysfin on the subcellular structures of C6 glioma ceils in vitro.Methods C6 glioma cells in vitro were treated with Lactacysfin.Photo-microseope was used to observe cell death,and transmission electron microscope were used to observe subcellular Structures＇ alteration. Results Lactacystin dealed with.C6 glioma cells in 24 h, kytoplasm was concentrated and anachomasised, the chromatin of nucleus was concentrated pyknoficly to see under photo-microscope. The microvilli on surface dispeared,nucleus crenated,chromafin aggregated pyknoticly under transmission electron microscope respectively. Conclusion Proteasome inhibitor Lactacystin could induce C6 glioma cells apoptosis in vitro.