甲基弯菌IMV 3011细胞生物催化二氧化碳制甲醇

Methanol Production from CO_2 by Resting Cells of Methylosinus trichosporium IMV 3011

甲基弯菌IMV 3011可以催化二氧化碳生物转化生成甲醇.在细胞悬浮液中充入二氧化碳后,反应一段时间后在反应液中检测到了甲醇产生.但是甲烷氧化细菌细胞合成甲醇的能力受到了细胞内还原当量的限制.研究发现,细胞内贮存的聚-β羟基丁酸(PHB)分解后能够产生还原当量,可以提高甲醇的产生能力.本文通过改变培养基中氮和铜的起始浓度对PHB积累量进行调节来提高甲基弯菌IMV 3011还原二氧化碳生成甲醇的能力.结果表明,随着细胞内PHB含量的增加甲醇的产生能力也会增加.当细胞内PHB的积累量达到38.6%时,将二氧化碳还原成甲醇的能力最强.当PHB的积累量超过38.6%时细胞生成甲醇的能力反而降低.

Methanol production from carbon dioxide was successfully achieved using resting cells of Methylosinus trichosporium IMV 3011 as biocatalysts. Carbon dioxide is reduced to methanol and extracellular methanol accumulation has been found in the carbon dioxide incubations. However, resting cells of methanotrophs have a finite or intrinsic methanol production capacity due to a limiting supply of intracellular reducing equivalent. It has been found that the catabolism of stored Poly-β-Hydroxybutyrate （PHB） can provide intracellular reducing equivalents to improve the intrinsic methanol production capacity. The initial concentration of nitrogen and copper in the culture medium were studied for the accumulation of PHB by M. trichosporium IMV 3011, to expand its potential uses in methanol production from carbon dioxide reduction. The results showed that the total methanol production capacity was improved with increasing PHB content in cells. Resting cells containing 38.6% PHB exhibited the highest total methanol production capacity. But higher PHB accumulation adversely affected the total methanol production capacity.