氟化物的抗雄激素样作用研究

The Experimental Study on the Anti-androgen effect of Fluoride

目的研究氟化物的抗雄激素样作用,并探讨其生殖系统损伤机制。方法采用Hershberger实验方法;同时应用放射免疫法检测血清睾酮含量。结果染毒组雄激素依赖组织(精液囊和凝集腺、前列腺、肛提肌/球海绵体肌、阴茎头、尿道球腺、阴茎)脏器系数及血清睾酮含量均高于阳性对照组和空白对照组,差异有统计学意义(P<0.05);染毒组雄激素依赖组织脏器系数与阴性对照组相比,差异无统计学意义(P>0.05)。低剂量染毒组血清睾酮含量高于阴性对照组,差异有统计学意义(P<0.05),中剂量和高剂量染毒组血清睾酮与阴性对照组相比,差异无统计学意义(P>0.05);各处理组之间肝、脾、肾、肾上腺脏器系数差异均无统计学意义(P>0.05)。结论氟化钠可能不具有抗雄激素样作用。

Objective To study the anti-androgen effect of fluoride,and approach the damage mechanism on the reproductive system.Methods Use the method of rat Hershberger Assay;and detect the level of blood serum testosterone by radio-immunity.Results The weight percentage of androgen related organs（seminal vesicle,prostate,LA/BC muscle,glans penis,Cowper＇s gland,penis）of NaF treated groups and the level of blood serum testosterone is higher than the positive control group and blank control group,the deficiencies were significant（P〈0.05）;There is essentially no differences in the indexes of weight percentage of androgen related organs between NaF treated groups and negative control group（P〉0.05）,the blood serum testosterone of low dose of NaF treated group is higher than the negative control group,the deficiencies were significant（P〈0.05）,and there are essentially no differences in the blood serum testosterone between middle and high dose NaF treated groups and negative control group;There have no significant deficiencies among the treatment groups＇ organs such as lives,spleens,kidneys and adrenal glands（P〉0.05）.Conclusions Sodium Fluoride may not have the anti-androgen activity.