卡氏肺孢子虫MSG抗原片段细胞表位的预测

PREDICTION OF CELL EPITOPES OF PARTIAL MSG ANTIGEN FRAGMENT OF PNEUMOCYSTIS CARINII

[目的]预测卡氏肺孢子虫主要表面抗原(MSG)片段的B细胞和T细胞抗原表位。[方法]运用Sig-nalp3.0、EMBOSS等网络服务器推测卡氏肺孢子虫MSG抗原片段的B细胞和T细胞抗原表位,分析预测结果。[结果]MSG抗原片段存在3个潜在的B细胞抗原表位及6个潜在的T细胞抗原表位。B细胞抗原表位在氨基酸序列的位置为69-79、96-101、133-140aa3个区域内或其附近;T细胞抗原表位在氨基酸残基的位置为7-22、28-43、20-35、40-55、53-68、86-101aa。[结论]MSG抗原表位的预测为有目的的克隆基因片段,研究高效的表位疫苗奠定基础。

[Objective] To predict B celt and T cell epitopes of surface antigen （MSG） fragment of Pneumocystis carinii. [Methods] The B cell and T cell epitopes for MSG antigen fragment of Pneumocystis carinii were predicted and analyzed by network servers including Signalp3.0, EMBOSS and so on. [Results] There were three potential epitopes of B cell and six potential epitopes of T cell in MS（; antigen. The epitopes of B cell located in the amino acid sequence of 69-79, 96-101 and 133-140aa or nearby. The epitopes of T cell located in amino acid sequence of 7-22, 28-43, 20-35, 40-55, 53-68 and 86- 101aa. [Conclusion] Prediction of the B cell and T cell epitopes for MSG antigen of Pneumocystis carinii would be helpful for purposely cloning gene fragment and establish basis for further researching on the high effectively epitope vaccine.