伊木萨克片对DM性ED大鼠外周血中性激素水平的影响

The effects of Yimusake tablet on the level of serum sex hormones in DM rats with ED

目的研究伊木萨克片对DM性ED大鼠外周血中性激素水平的影响。方法取雄性SD大鼠70只,从中随机取10只为正常对照组,余60只以链脲佐菌素诱导建立DM模型后,行阿朴吗啡阴茎勃起实验筛选DM性ED模型。发生ED者随机分为DM性ED组、伊木萨克片组、胰岛素组、伊木萨克片+胰岛素(联用)组,未成DM者为STZ组,各组给药6周后,采用放射免疫法检测各组大鼠外周血中睾酮(T)、促黄体生成素(LH)和促卵泡刺激素(FSH)水平,同时称取睾丸重量,镜检睾丸的组织形态学改变。结果DM性ED组睾酮水平显著低于正常对照组(P<0.01);伊木萨克片组、胰岛素组与联用组睾酮水平显著高于DM性ED组(P<0.01,P<0.01,P<0.01);联用组睾酮水平显著高于伊木萨克片组与胰岛素组(P<0.01,P<0.01);DM性ED组LH水平显著高于正常对照组(P<0.01),FSH水平在各组之间差异均无统计学意义(P>0.05)。HE结果显示:DM性ED组生精小管发生显著病理变化,生精细胞、间质细胞数量较正常对照组和STZ组明显减少,各治疗组则明显好转,其中联用组显微结构与正常对照组相似。结论(1)DM可致睾丸结构改变,影响睾酮的合成与分泌,并致大鼠血清睾酮水平显著降低;(2)伊木萨克片可显著提高DM性ED大鼠外周血中睾酮水平,在胰岛素控制血糖的基础上效果可能更佳,并提示伊木萨克片治疗DM性ED的作用机制可能与提高睾酮水平有关。

Objective To explore the effect of Yimusake tablet on the level of serum sex hormones in DM rats with ED. Methods Seventy male sprague-dwaley rats were used for the study, Ten of them were randomly selected for the control group（NDM）.The other sixty rats were injected with streptozotozin （STZ） for estabilishment of diabetes mellitus （DM） model, and then all of them were injected with apomorphine （APO） to test their erectile function. The ED rats were devided into four groups including the model （ED with DM） group, the Yimusake group, the insulin group and the Yimusake with insulin group.The rats with no diabetes were enrolled in the STZ group. After drug treatment for Six weeks later.the level of serum testosterone, luteinizing hormone （LH） and follicle-stimulating hormone （FSH） of all groups were detected by radioimmunoassay and the weight of the testitulars were measured and the morphological changes were observered in testicular tissue under microscope. Results The level of testosterone in ED with DM group was lower than that of the NDM group （P〈0.01）; The level of testosterone in Yimusake group, insulin group and Yimusake with insulin group were higher than that in the ED with DM group （P〈0.01, P〈0.01, P〈0.01）; The level of testosterone in Yimusake and insulin group was higher than that in the Yimusake group and insulin group （P〈0.01, P〈0.01）; The level of luteinizing hormone （LH） in ED with DM group was higher than thatin the NDM group （P〈0.01）.There was no significant in the level of folliclestimulating hormone （FSH） （P〉0.05）. HE staining of testicular tissue showed that an obvious pathological changes seminiferous tubules were found in ED with DM group.And the number of spermatogetic cells and interstitial cells in DM group with ED decreased in compared with that of the NDM group and STZ group.Rats in all treatment groups improved a lot. There was a similar microstructure between the NDM group and the yimusake with insulin group. Conclusion （1） DM might cause structure change in testitulars,affect synthesis and secretion of testosterone and downregulate the level of serum testosterone of rats. （2）Yimusake tablet has significantly increased the level of serum testosterone in ED with DM rats, and have a better effect in ED treatment under the controlling blood sugar with insulin. So it indicated that the mechanism of Yimusake tablet treated ED with DM may be related to the raise of testosterone level.