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牛体细胞中Gadd45a的表达与DNA甲基化修饰 被引量：2

DNA Methylation and Regulation of Gadd45a Expression in Bovine Adult Tissues
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摘要 目的检测成年牛心脏、肾脏、肝脏及睾丸组织中Gadd45a基因表达情况及DNA甲基化状态,并说明二者之间的关系。结果牛Gadd45a基因在心脏中没有表达,而在其他3种组织中有表达且略有差异。选定该基因调控区中的7个CpG位点,利用甲基化特异性PCR检测CpG位点的DNA甲基化状态,结果心脏中的甲基化程度明显高于其他3种组织。结论DNA甲基化对牛Gadd45a基因的组织特异性表达有一定影响。 Aim To examine the transcription of Gadd45a and the status of DNA methylation in adult bovine tissues, and analyze the relationship between them. Result The Gadd45a mRNA was not detected in heart but was found in the other three tissues （kidney, liver and testicle） with different levels. 7 CpG sites in the regulation region of Gadd45a were chosen to examine their DNA methylation status by the methylated specific PCR. The result showed that the methylation level in heart was obviously higher than in the other three tissues. Conclusion The expression of bovine Gadd45a is related to DNA methylation.
作者 李轲涵 岳永莉 辛鹏慧 于海泉
机构地区 内蒙古大学
出处 《四川动物》 CSCD 北大核心 2008年第5期794-796,共3页 Sichuan Journal of Zoology
基金 教育部博士点基金项目(205229) 内蒙古自然科学基金重点项目(205184)
关键词 牛 正常体细胞 DNA甲基化 cattle adult somatic cell DNA methylation
分类号 Q78 [生物学—分子生物学]
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参考文献13

  • 1宫时玉,蒋曹德,邓昌彦.DNA甲基化及其生物学功能[J].华中农业大学学报,2005,24(6):651-657. 被引量：14
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二级参考文献19

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四川动物
2008年 第5期

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