端粒酶逆转录酶在大鼠肝癌发生中的表达变化

Expression Level of Telomerase Reverse Transcriptase in Development of Rat Hepatocellular Carcinoma

端粒酶逆转录酶(telomerase reverse transcriptase,TERT)在永生化及肿瘤细胞中表达升高而被认为是调控端粒酶活性的限速步骤。通过二乙基亚硝胺(diethyl nitrosoamine,DENA)诱导大鼠肝细胞肝癌(hepatocellular carcinoma,HCC)的发生,采用免疫荧光和Western blot方法检测TERT蛋白在肝细胞内的表达变化,发现对照组及造模组的炎症期和癌旁组织中TERT蛋白的表达较低并维持在相对稳定的水平。随着病变的进展,TERT的表达在肝硬化期急增并在其后的癌发生过程中持续保持这一高水平。TERT蛋白的表达升高与肝癌的发生发展相一致。

Telomerasc reverse transcriptase （TERT） expression, which is closely associated with cell immortalization and carcinogenesis, is regarded as a rate-limiting factor in the regulation of telomerase activity. The expression of TERT was detected using Western blot and immunoiluorescence analysis, respectively, in rat hepatoeellular carcinoma （HCC） model induced by diethyl nitrosoamine （DENA）. The low expression level of TERT was detected and maintained at a relative stable level in normal tissue and normal tissue adjacent to tumor, whereas the expression of TERT was significantly increased in hepatocirrhosis stage, especially in HCC stage. Therefore, over-expression of TERT was consistent with the progression of HCC.