高效液相色谱法测定防己药材中粉防己碱与防己诺林碱的含量

Determination of tetrandrine and fangchindine in Stephania tetradra S. Moor by HPLC

目的用高效液相色谱法测定不同产地防己药材中粉防己碱与防己诺林碱的含量。方法色谱柱为Kromasil ODS-1（4．6mm×200mm，5μm），流动相为乙腈0．01％三乙胺水溶液，梯度洗脱，流速为1．0mL·min^-1，检测波长为280nm。结果粉防己碱在474-4740ng内线性关系良好，r=0．9999，平均回收率为99．3％，RSD=1．5％；防己诺林碱在238-2380ng线性关系良好，r=0．9998，平均回收率为99．5％，RSD=1．1％。结论建立了高效液相色谱法同时测定防己药材中粉防己碱与防己诺林碱的含量，比较了不同产地防己药材中粉防己碱与防己诺林碱的含量。

Objective To established a method for the determination of tetrandrine and fangchindine in Stephania tetradra S. Moore from different areas by HPLC. Methods The separation was performed on a Kromasil ODS-1 （4.6 mm× 200 mm, 5 μm） . ACN-0.01% triethylamine was used as the mobile phase with gradient elution. The flow rate was 1.0 mL · min^- 1. The UV detection wavelength was 280 nm. Results The calibration curves of tetrandrine were in good linearity over 474-4 740 ng （r=0. 999 9）. The average recovery was 99.3%, and RSD was 1.5%. The calibration curves of fangchindine were in good linearity over 238- 2 380 ng （r= 0. 999 8）. The average recovery was 99.5%, and RSD was 1.1%. Conclusion An HPLC method for the quantitative determination of tetrandrine and Fangchindine in stephania in stephania tetradras, moore was established. And the contents of tetrandrine and Fangchindine in Stephania tetradra S. Moor from different places were markedly different.