不同材料包埋固定化厌氧氨氧化混培物

Nitrogen removal with ANAMMOX mixed culture immobilized in different materials

为探寻一条维持反应器中厌氧氨氧化混培物生物量的新途径,采用CMC、PVA、SA以及PVA-SA混合液等为包埋材料,对厌氧氨氧化混培物进行包埋固定,制成固定化小球.结果表明,4种包埋固定化小球均表现出较高的厌氧氨氧化活性,氨氮和亚硝酸盐氮的去除率分别达到100%和96%-98%,其中氨氮、亚硝酸盐氮去除量和硝酸盐氮生成量的比值在1∶（1.142-1.252）∶（0.200-0.365）之间.4种包埋固定化小球的厌氧氨氧化活性排序为CMC小球〉SA小球〉PVA-SA小球〉PVA小球.机械性能排序为PVA-SA小球〉PVA小球〉SA小球〉CMC小球.PVA小球粘连现象严重,成球效果最差.综合评价PVA-SA为最佳的固定化材料.

Four different materials,Sodium Carboxymethylcellulose（Na-CMC）,Sodium Alginate（SA）,Polyvinyl Alcohol（PVA）and PVA-SA mixed liquor were selected as supports to entrap Anaerobic Ammonia Oxidation（ANAMMOX）mixed culture.The nitrogen removal by the immobilized gel beads and plain anammox mixed culture were evaluated.The results showed that the average removal ratios of ammonium and nitrite with immobilized gel beads were 100% and 97%,respectively in 49 hours.The ratio of nitrite nitrogen consumed to ammonium nitrogen consumed to nitrate nitrogen produced was 1∶（1.142-1.252）∶（0.200-0.365）.Thus,use of immobilized anammox culture in gel beads is feasible.The sequence of anammox activities were CMC〉SA 〉PVA-SA 〉PVA.Subsequently,their physical characteristics were measured,showing that PVA-SA beads had the best mechanical strength and physical stability.PVA-SA was the best support material among the four,considering immobilization characteristics,removal ratio,and cost of the materials.