摘要
目的卵黄蛋白原(vitellogenin,VTG)可以作为检测环境雌激素生物标志物,用于评价化学物质雌激素能力或动物在存在有雌激素污染物水体中的暴露状况。该文主要研究壬基酚(nonylphenol,NP)对河川沙塘鳢幼鱼VTG的诱导的情况。方法把孵化出的河川沙塘鳢仔鱼暴露于壬基酚中,设立3个浓度(100、300和500μg/L),同时设空白对照和溶剂对照,暴露时间为150 d,然后取样进行检测。结果与对照相比,VTG蛋白的含量以及基因的表达随着NP浓度的增加而递增,具有明显的浓度效应,并且在NP 500μg/L时,差异具有统计学意义(P<0.01)。结论可以用VTG的含量及基因表达水平作为监测环境雌激素类化学物暴露有效的终点指标。
[ Objective] Vitellogenin （ VTG） , which can be used as a biomarker for assessing the environmental estrogens, was used to evaluate the ability of estrogen chemicals or exposure situation of animals in the presence of estrogen pollutants in water. This paper mainly studied the effect of nonyl phenol （ NP） on VTG inducing in odontobutis potamophila. [ Methods] The larva were ex- posed to nonyl Phenol, 3 concentration （100, 300 and 500 μg/L） was established, setting up blank control group and solvent control, after 150 d exposure, samples were collected for testing. [ Results] Compared with control group, VTG protein content and gene expression increased with the NP concentration, and there was apparent concentration effect, and when NP level was 500 μg/L, the difference was significant. [ Conclusion] The concentration of VTG and its gene expression level can be the appropriate endpoints for screening environmental estrogens.
出处
《职业与健康》
CAS
2008年第20期2125-2127,共3页
Occupation and Health
关键词
壬基酚
河川沙塘鳢
卵黄蛋白原
Nonyl phenol
Odontobutis potamophila
Vitellogenin
