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用单细胞凝胶电泳方法评价辐射非靶效应研究

Study on the Non-target Effect of Ionizing Radiation Using Single Cell Gel Electrophoresis
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摘要 目的用单细胞凝胶电泳的方法评价电离辐射的非靶效应。方法将人外周血自体受照淋巴细胞及未受照淋巴细胞分别与自体受照血浆及未受照血浆交叉混合孵育,用单细胞凝胶电泳法(single cell gel electrophoresis,SCGE)分析其DNA损伤程度。结果自体未受照淋巴细胞与自体受照血浆共同孵育后,其淋巴细胞的DNA损伤明显高于自体未受照淋巴细胞与自体未受照血浆共同孵育后淋巴细胞DNA的损伤(P<0.05)。结论单细胞凝胶电泳技术可以用来评价辐射的非靶效应,并进一步证实了在辐射的非靶效应中细胞因子可能起到非常重要的作用。 Objective To assess the non - target effect of ionizing radiation by single cell gel electrophoresis （SCGE）. Methods Cross incubated the irradiated（ 137 Cs; 2Gy） or non - irradiated lymphocytes of human peripheral blood in the irradiated or non - irradiated plasma respectively, then, assess the DNA damage of lymphocytes using SCGE analysis. Results The lymphotytes incubated in the irradiated plasma presented more obvious DNA damage than the incubated in the non - irradiated plasma dose （ P 〈 0.05 ）. Conclusion The non - target effect of ionizing radiation can be assessed by SCGE, and the results confirm that cytokines may play a great role in it.
作者 王彦 李德冠 刘鉴峰 褚丽萍 刘强
机构地区 中国医学科学院放射医学研究所
出处 《中国辐射卫生》 北大核心 2008年第3期257-258,共2页 Chinese Journal of Radiological Health
基金 天津市自然科学基金项目(编号:07CYBJC09200)
关键词 单细胞凝胶电泳 电离辐射 非靶效应 Single Cell Gel Electrophoresis Irradiate Non - Target Effect
分类号 R811.5 [医药卫生—放射医学]
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参考文献10

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二级参考文献8

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共引文献12

中国辐射卫生
2008年 第3期

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