摘要
目的通过检测不同剂量电离辐射对大肠癌细胞多药耐药基因MDR1的蛋白表达产物P-糖蛋白(P-gp)的影响,探讨逆转肿瘤多药耐药的方法。方法采用流式细胞术检测P-gp蛋白表达的变化。结果与假照组相比,2Gy大剂量照射后P-gp阳性细胞百分率明显增加(P<0.01),先给予低剂量照射(0.05Gy,0.1Gy)后,再给予大剂量照射,P-gp阳性细胞百分率亦有明显增加(P<0.05),0.2Gy+2Gy组P-gp阳性细胞百分率明显增加(P<0.01)。与单纯2Gy大剂量照射组比较,0.1Gy+2Gy组P-gp阳性细胞百分率明显降低(P<0.05)。结论低剂量辐射可以逆转大剂量辐射所致的多药耐药性。
Objective To detect the method of reversing tumor multidrug resistance, and study the expression of colorectal carcinoma MDR1 genetic expression product P - glycoprotein （P -gp） in irradiated HCT- 8 cells of with different dose ionizing radiation. Methods Flow cytometry was used to detect the change of P - gp. Results positive cells percentage of P-gp increase obviously（ P 〈 0.01 ） after irradiated with 2Gy. When pre - irradiated with low dose （0.05Gy,0.1 Gy） , then irradiated with high dose, positive cell percentage of P-gp increase obviously（ P 〈 0.05 ）, and so dose the positive cell percentage of P- gp （P 〈0.01 ）in the group of 0.2Gy ＋ 2Gy. Compared with the group of 2Gy, positive cell percentage of P - gp decreases obviously （ P 〈 0.05 ） in the group of 0.1 Gy ＋ 2Gy. Couclusion low dose ionizing radiation can reverse radiogenic tumor multidrug resistance with high dose.
出处
《中国辐射卫生》
北大核心
2008年第3期277-278,共2页
Chinese Journal of Radiological Health
关键词
辐射
大肠癌
多药耐药
P-糖蛋白
Rradiation
Colorectal Carcinoma
Muhidrug Resistance
P - Glycoprotein
