电磁辐射对家兔运动性学习记忆能力及小脑组织形态结构的影响

Effects on Motor Learning and Pathological Histology in Rabbit Cerebellum by Electromagnetic Irradiation

目的观察电磁辐射对家兔运动性学习记忆功能及小脑组织形态结构的影响。方法选择建立牢固瞬膜条件反射的家兔,随机分为对照组和电磁辐射组(包括辐射后0、3、24和72h等四个时相点),辐射组给予平均功率密度90mW/cm2的电磁辐射30min,测定辐射后家兔的即刻肛温并计算比吸收率(SAR)值;检测辐射后各时相点家兔瞬膜条件反射和小脑组织形态结构的变化。结果90mW/cm2电磁辐射后即刻家兔肛温升高2.98℃,SAR值为5.75kcal/kg;瞬膜条件反射在辐射后0h显著降低,3h后基本恢复至正常水平;小脑组织形态结构损伤以蒲肯野细胞萎缩变形和颗粒细胞排列紊乱为主。结论90mW/cm2电磁辐射30min能使家兔机体产生明显热效应,并损害小脑的组织形态结构和运动性学习记忆功能。

Objective To investigate the effects of electromagnetic irradiation on motor learning and pathological histology in rabbit cerebellum. Methods To train rabbits for seven days to form eye blink conditioning, and then rabbits were divided randomly into control and electromagnetic irradiation group （including 0 hour, 3 hours, 24 hours and 72 hours subgroups after irradiation）, rabbits were given 90 mW/cm^2 electromagnetic irradiation for 30 minutes, rectal temperature were detected immediately after irradiation and specific absorbtion rate（SAR） value were calculated, eye blink conditioning were detected immediately after irradiation, cerebellar pathological histology were detected with hematoxylin and eosin（HE） stain. Resuits Rectal temperature of rabbits increased 2.98 ℃ after electromagnetic irradiation, SAR value is 5.75 Kcal/kg , eye blink conditioning decreased significantly after irradiation, the changes of cerebellar pathological histology were party of purkin- je cells depauperate and granule cells arranged disorderly. Conclusion After 90 mW/cm^2 electromagnetic irradiation for 30 min, heating effect occured obviously in the rabbits and their cerebellum motor learning and pathological histology were endamagered seriously.