电磁辐射对子代大鼠学习记忆功能的影响

Effects of electromagnetic radiation exposure on learning and memory function of rat pups

目的研究电磁辐射对子代大鼠学习记忆能力的影响。方法健康Wistar大鼠分为雌雄2组，每组又随机分为4组：对照组（CRT）、100s组（SI）、1000s组（S10）和3000s组（S30）组，分别接受0s、100s、1000s和3000s电磁辐射（场强100kV／m）。辐照后2月将辐照时间相同的雌性和雄性动物合笼生育子代，子代雌雄2组根据其亲代的分组方式相应分为F—CTR、F—S1、F—S10、F-S30（F指子代），各4组，每组20只。通过行为学实验检测2月龄和6月龄子代大鼠的学习记忆能力。结果（1）2月龄子代大鼠：穿梭实验中各辐照组与对照组比均差异无显著性。Y迷宫中雄性F—S10与雄性F—CTR比，学习总次数和错误次数都减少，学习总次数为（14．6±3．9）次vs（21．1±7．8）次，错误次数为（3．5±2．4）次vs（7．8±5．4）次（P〈0．05）。雌性F—S10与雌性F—CTR比，学习总次数和错误次数也都减少，分别为（13．4±3．0）次vs（25．8±8．8）次，（3．4±2．6）次vs（11．0±7．2）次（P〈0．05）。旷场实验中雌雄2组的F—S1与其对应的F—CTR，自发活动和探索能力都提高（P〈0．05）。（2）6月龄子代大鼠：Y迷宫中仅雄性组中的F—S1和F-S30与F—CTR比学习总次数增加，分别为（24．2±8．9）次vs（14．1±5．2）次，（30.7±12．4）次vs（14．1±5．2）次，（P〈0．05）。穿梭试验和旷场实验中各辐射组与对照组比均差异无显著性。结论在该辐照条件下，100kV／m的电磁辐射对2月子代大鼠记忆及对新事物的探索能力表现出刺激效应，成年后这种影响减弱。

Objective To determine whether exposure to electromagnetic radiation results in behavioral effects of rat pups. Methods Wistar rats, either male or female, were divided randomly into four groups respectively： control （CTR）, 100 seconds （ S1 ）, 1000 seconds （ S10 ） and 3000 seconds （ S30 ）, then each of them were exposed to electromagnetic radiation of 100 kV/m field amplitude of corresponding time . For the day of exposure till 2 months later,they were mated with the rats in the same group and their offspring were divided into four groups （ F-GTR, F-S1, F-S10, F-S30） correspondingly. Behavioral changes occur in 2-month old and 6-month old rat pups were performed to determine. Results （1）In 2-month-old rat pups ： in step through test, no significant differences were found between radiation groups and control, and in Y-maze test, both male and female pups in F-S10 significantly learned fewer times than their control （total study times：male 14.6 ± 3.9 vs 21.1 ± 7.8,female13.4 ± 3.0 vs 25.8 ± 8.8 ; false times ： male 3.5 ± 2.4 vs 7.8 ± 5.4, female 3.4 ± 2.6 vs 11.0 ± 7.2）. In open field test, both male and female pups in F-S1 was significantly more activity than their control. （2）In 6-month-old rat pups：in Y maze,male pups in F-S1 and F-S30 learned more times than control in total study time （24.2 ± 8.9 vs 14.1 ± 5.2,30.7 ± 12.4 vs 14.1 ± 5.2）. In step through test and open field test, no significant differences were found between radiation groups and control. Conclusion There was significant genetic effect exposed to electromagnetic radiation of 100 kV/m,mainly manifested in rat pups in growth period but without long-term effect.