白首乌提取物对血管性痴呆模型小鼠学习记忆功能的影响

Effects of Radix Cynanchum auriculatum extract on learning and memory deficits in mice with vascular dementia

目的研究自首乌提取物对血管性痴呆模型小鼠学习记忆功能和抗氧化水平的影响。方法小鼠反复脑缺血再灌注合并尾部放血降压建立痴呆模型；Morris水迷宫观察各组小鼠的学习记忆功能；快速断头处死，观测张口喘气时间；测定海马区及大脑皮质组织匀浆中超氧化物歧化酶（SOD）、丙二醛（MDA）水平。结果与模型组相比，药物高剂量组逃避潜伏期缩短，差异具有显著性（P〈0．01），正确探索时间延长（P〈0．01）；张口喘气时间延长[（19．3±1．6）svs（15．7±0．7）s，P〈0．01]；SOD活性升高[（121．0±14．2）U·mgprot^-1vs（101．5±9．4）U·mgprot^-1，P〈0．05]；MDA含量降低[（5．12±1．09）nmol·mgprot^-1和（6．77±0．96）nmol·mgprot^-1，P〈0．01]。结论自首乌提取物对小鼠脑缺血再灌注损伤有保护作用，其机制可能与抗自由基损伤有关。

Objective To observe the effects of Radix Cynanchum anriculatum extract in mice with vascular dementia. Methods An animal model was established through repeated cerebral isehemia-reperfusion in mice with tail bleeding hypotension. Morris water-maze was used to determine the learning and memory ability; gasping time of mice by decapitation were evaluated. Changes in superoxide dimutase （SOD）, malonaldehyde （MDA） were observed by means of spectro photometry. Results To contrast high dose drug group with model group, the escape latencies were significantly shorter （P 〈 0.01 ） , contrarily the time in correct zone were prolonged （P 〈 0. O1 ）. And the gasping time of mice by decapitation were also protracted [ （ 19.3± 1.6） s vs （ 15.7 ± 0.7） s ] （P 〈 0.01 ）. The high dose group had less content of MDA [ （5.12± 1.09） nmol · mgprot^-1 vs （ 6.77 ± 0.96） nmol · mgprot^-1 ] （P 〈 0.01 ）, but more activity of SOD in hippoeampus and cerebral cortex [ （ 121.0 ± 14.2） U · mgprot ^-1 vs （ 101.5 ± 9.4 ） U · mgprot ^-1] （P〈 0.05）. Conclusion Radix Cynanchum auriculatum extract has protective effect on cerebral ischemia-reperfusion injury.. The mechanism may be related with anti-oxidation and free radical.