5-羟色胺转运体基因多态性与难治性强迫症的关联分析

Associate analysis of 5-HTTLPR polymorphism and refractory obsessive-compulsive disorder

目的探讨汉族人群中难治性强迫症与非难治性强迫症的5-羟色胺转运体启动子区（5-HT—TLPR）基因多态性差异。方法采用聚合酶链反应（PCR）和限制性片断长度多态性（RFLP）技术，检测51例难治性强迫症和59例非难治性强迫症的5-HTT基因型，并比较2组之间的5-HTT基因多态性差异。结果难治性强迫症和非难治性强迫症组5-HTT的基因型（χ^2=11．09，P=0．02）和等位基因频率（χ^2=20．04，P=0．00）差异有显著性。L^＋／L^＋基因型和L^＋等位基因与难治性强迫症呈明显正关联（OR=9．39，χ^2=9．51，P〈0．01；OR=23．40，χ^2=19．03，P〈0．01。结论5-HTT基因多态性中L^＋／L^＋基因型和L^＋与难治性强迫症相关联，是难治性强迫症的危险因子。

Objective To explore the difference of 5-HTTLPR polymorphism between refractory and nonrefractory obsessive-compulsive disorder （OCD） in the Han nationality. Methods 51 refractory OCD patients and 59 non-refractory OCD patients were took part in this study. The gene polymorphism of 5-HTT in all subjects was genotyped directly via polymerase chain reaction（PCR） and restriction fragment length polymorphism（RFLP） technique, and to compare the differences of 5-HTT polymorphism between refractory and non-refractory OCD. Results There was significant difference between refractory and non-refractroy OCD patients in the frequencies of genotype （χ^2 = 11.09, P = 0.02） and allele （χ^2 = 20.04, P = 0.00） for 5-HTTLPR ; L ^＋ / L ^＋ genotype and L ^＋ Allele of 5-HTT was positively associated with OCD （OR = 9.39, χ^2 = 9.51, P 〈 0.01 ; OR = 23.40, χ^2 = 19.03, P 〈 0.01 ）. Conclusion L ^＋/ L ^＋ genotype and L ^＋ allele of 5-HTT gene may be associated with OCD in the HaM nationality, may be risk factor for refractory OCD.