两种结核抗体金标试剂的应用评价

Evaluation of application of two golden-labelled reagents for detection of antibody to Mycobacterium tuberculosis

目的评价两种结核抗体金标试剂的应用价值。方法选取痰检阳性结核病患者50例,健康成人对照25例及接种卡介苗儿童25例,分别平行用上海奥普及新加坡MP公司生产的结核抗体金标试剂进行检测,比较两种试剂检测结果。结果上海奥普结核抗体金标试剂检测的敏感度为96.0%(48/50),特异度为92.0%(23/25),卡介苗接种组阳性率8.0%(2/25);新加坡MP结核抗体金标试剂检测的敏感度为80.0%(40/50),特异度为88.0%(22/25),卡介苗接种组阳性率68.0%(17/25),两种试剂的敏感度及卡介苗接种后阳性率差异有显著性(P<0.05),但两种试剂的特异性无统计学差异(P>0.05)。结论结核抗体金标试剂操作简单,使用方便,但不同试剂的应用价值差异大,应对其评价后方可选用。

Objective To assess the value of two golden - labelled reagents in detection of antibodies to Mycobatrium tubercuosis. Methods There 50 supntum positive patients, 25 healthy adults and 25 children vaeeinated with BCG were selected and antibodies to Mycobacterium tuberculosis were detected with golden - labelled reagents produeed in Shanghai Aopu Company and MP of Singapore. Results The sensitivity and specificity of test with Shanghai Aopu reagent was 96. 0% （48/50） and 92.0% （23/25）, the positive rate of BCG vaecination group was 8% （2/25） ; The sensitivity and specifieity of Singaporian MP reagent was 80.0% （40/50 and 88% （22/25）, While the positive rate of BCG vaccination group was 68% （17/25）. The sensitivity of the two reagents were significantly different （P 〈 0.05）, but not the specificity （P 〉 0.05 ）. Conclusion The method of detection of antibody to Mycobacterium tuberculosis with the reagents is simple and convenient, but there is variations and assessment be made before application.