耐红霉素葡萄球菌中克林霉素诱导型耐药株的检测分析

Detection of clindamycin-induced ressitance of erythromycin-resistant bacteria

目的了解本院临床分离的葡萄球菌中克林霉素诱导型耐药的发生率,帮助临床医师正确选用药物。方法采用K-B纸片琼脂扩散法检测葡萄球菌对红霉素和克林霉素的耐药性,按照CLSI/NCCLS推荐的D-试验方法检测葡萄球菌的克林霉素诱导型耐药。结果耐甲氧西林金黄色葡萄球菌(MRSA)和耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)对红霉素及克林霉素同时耐药分别占61.53%和37.03%。D-试验阳性菌株占所检测葡萄球菌的19.16%,占红霉素耐药而克林霉素敏感菌株的52.87%。对红霉素耐药而克林霉素敏感的金黄色葡萄球菌和凝固酶阴性葡萄球菌中,D-试验阳性者分别为64.28%和47.45%。结论收集的葡萄球菌中克林霉素诱导型耐药株的发生率较高,应引起临床医生的高度重视。

Objective To understand the incidence of elindamyein - induced resistance in clinical strains of Staphylococcus. Methods To dermine the resistance of Staphylocococcus to erythromein and clindamyein by K - B agar diffusion method and the elindamyein - induced resistance by D - test recommended by CLSI/NCCLS. Results There 61. 53% of MRSA and 37. 03% of MRCNS were resistant to erythromein and clindamycin. There 19. 16% of detected Staphylococcus were positive by D - test, and 52.87% of erythromcin - resistant Staphylococcus were positive. The positive rates of D - test of Staphylococcus aureus resistant to erythromcin and sensitive to clindamyein and and negative to eoagalase were 64.28% and 47.45% respectively. Conclusion The rate of e/indamycin - induced resistance is high in this hospital and reasonable and proper use of antibiotics for clinical treatment is indicated.