肥大细胞三种染色法的比较研究

CONTRAST ANALYSIS OF THREE STAININGS OF MAST CELLS

目的对肥大细胞的三种染色方法比较，筛选出最佳显示方法。资料与方法，选用手术切除的消化道恶性肿瘤标本９４例，应用硫堇、甲苯胺蓝和甲基绿一派洛宁三种染色法显示肥大细胞，进行同一组织切片分组染色，镜下观察比较分析。结果甲基绿一派洛宁染色法显示肥大细胞效果最佳，此方法除可分别显示出幼稚的肥大细胞和成熟的肥大细胞外，还能清晰地显示出肿瘤细胞及其间质组织等其它的组织成分。结论甲基绿一派洛宁染色法其组织结构清晰，色彩鲜艳，对比度强。适用于组织形态学研究和临床外检的辅助诊断。

Objective To scveen out the best straining of mast cells. Methods The mast cells in the tumour specimens from 94 cases with gastrointestinal tract malignant tumour were stained with Thioine, Toluidine Blue and Methyl Green-Pyronine. Results Methyl Green-Pyronine staining was the best among the three stainings. Besides premature and mature mast cells. the tumour cells and insterstitial tissue can also be displayed clearly. Conclusion Methyl Green-Pyronine staining can display structure clearyl, colour Brightly and lustrously, and contrast strikingly It can be used on hostological and morpholoical study and as auessory examination to clinical biopsy.